بسم الله الرحمن الرحيم
2017-03-25 16:59:24 dun: Mahammad A. Tafida
Development and In Vitro Evaluation of Liposomes Using Soy Lecithin to Encapsulate Paclitaxel.
Int J Biomater. 2017;2017:8234712
Authors: Nguyen TL, Nguyen TH, Nguyen DH
The formulation of a potential delivery system based on liposomes (Lips) formulated from soy lecithin (SL) for paclitaxel (PTX) was achieved (PTX-Lips). At first, PTX-Lips were prepared by thin film method using SL and cholesterol and then were characterized for their physiochemical properties (particle size, polydispersity index, zeta potential, and morphology). The results indicated that PTX-Lips were spherical in shape with a dynamic light scattering (DLS) particle size of 131 ± 30.5 nm. Besides, PTX was efficiently encapsulated in Lips, 94.5 ± 3.2% for drug loading efficiency, and slowly released up to 96 h, compared with free PTX. More importantly, cell proliferation kit I (MTT) assay data showed that Lips were biocompatible nanocarriers, and in addition the incorporation of PTX into Lips has been proven successful in reducing the toxicity of PTX. As a result, development of Lips using SL may offer a stable delivery system and promising properties for loading and sustained release of PTX in cancer therapy.
PMID: 28331495 [PubMed - in ...
Fabrication of human hair keratin/jellyfish collagen/eggshell-derived hydroxyapatite osteoinductive biocomposite scaffolds for bone tissue engineering: From waste to regenerative medicine products.
Colloids Surf B Biointerfaces. 2017 Mar 18;154:160-170
Authors: Arslan YE, Sezgin Arslan T, Derkus B, Emregul E, Emregul KC
In the present study, we aimed at fabricating an osteoinductive biocomposite scaffold using keratin obtained from human hair, jellyfish collagen and eggshell-derived nano-sized spherical hydroxyapatite (nHA) for bone tissue engineering applications. Keratin, collagen and nHA were characterized with the modified Lowry method, free-sulfhydryl groups and hydroxyproline content analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) and thermal gravimetric analysis (TGA) which confirmed the success of the extraction and/or isolation processes. Human adipose mesenchymal stem cells (hAMSCs) were isolated and the cell surface markers were characterized via flow cytometry analysis in addition to multilineage differentiation capacity. The undifferentiated hAMSCs were highly positive for CD29, CD44, CD73, CD90 and CD105, but were not seen to express hematopoietic cell surface markers such as CD14, CD34 and CD45. The cells were successfully directed towards osteogenic, chondrogenic and adipogenic lineages in vitro. The microarchitecture of the scaffolds and cell attachment were evaluated using scanning electron microscopy (SEM). The cell viability on the scaffolds was assessed by the MTT assay which revealed no evidence of cytotoxicity. The osteogenic differentiation of hAMSCs on the scaffolds was determined histologically using alizarin red S, osteopontin and osteonectin stainings. Early osteogenic differentiation markers of hAMSCs were significantly expressed on the ...
Humanized mouse model for assessing the human immune response to xenogeneic and allogeneic decellularized biomaterials.
Biomaterials. 2017 Mar 11;129:98-110
Authors: Wang RM, Johnson TD, He J, Rong Z, Wong M, Nigam V, Behfar A, Xu Y, Christman KL
Current assessment of biomaterial biocompatibility is typically implemented in wild type rodent models. Unfortunately, different characteristics of the immune systems in rodents versus humans limit the capability of these models to mimic the human immune response to naturally derived biomaterials. Here we investigated the utility of humanized mice as an improved model for testing naturally derived biomaterials. Two injectable hydrogels derived from decellularized porcine or human cadaveric myocardium were compared. Three days and one week after subcutaneous injection, the hydrogels were analyzed for early and mid-phase immune responses, respectively. Immune cells in the humanized mouse model, particularly T-helper cells, responded distinctly between the xenogeneic and allogeneic biomaterials. The allogeneic extracellular matrix derived hydrogels elicited significantly reduced total, human specific, and CD4(+) T-helper cell infiltration in humanized mice compared to xenogeneic extracellular matrix hydrogels, which was not recapitulated in wild type mice. T-helper cells, in response to the allogeneic hydrogel material, were also less polarized towards a pro-remodeling Th2 phenotype compared to xenogeneic extracellular matrix hydrogels in humanized mice. In both models, both biomaterials induced the infiltration of macrophages polarized towards a M2 phenotype and T-helper cells polarized towards a Th2 phenotype. In conclusion, these studies showed the importance of testing naturally derived biomaterials in immune competent animals and the potential of utilizing this humanized mouse model for further studying human immune cell responses to ...
Cell survival and differentiation with nanocrystalline glass-like carbon using substantia nigra dopaminergic cells derived from transgenic mouse embryos.
PLoS One. 2017;12(3):e0173978
Authors: Rodriguez-Losada N, Romero P, Estivill-Torrús G, Guzmán de Villoria R, Aguirre JA
Regenerative medicine requires, in many cases, physical supports to facilitate appropriate cellular architecture, cell polarization and the improvement of the correct differentiation processes of embryonic stem cells, induced pluripotent cells or adult cells. Because the interest in carbon nanomaterials has grown within the last decade in light of a wide variety of applications, the aim of this study was to test and evaluate the suitability and cytocompatibility of a particular nanometer-thin nanocrystalline glass-like carbon film (NGLC) composed of curved graphene flakes joined by an amorphous carbon matrix. This material is a disordered structure with high transparency and electrical conductivity. For this purpose, we used a cell line (SN4741) from substantia nigra dopaminergic cells derived from transgenic mouse embryos. Cells were cultured either in a powder of increasing concentrations of NGLC microflakes (82±37μm) in the medium or on top of nanometer-thin films bathed in the same culture medium. The metabolism activity of SN4741 cells in presence of NGLC was assessed using methylthiazolyldiphenyl-tetrazolium (MTT) and apoptosis/necrosis flow cytometry assay respectively. Growth and proliferation as well as senescence were demonstrated by western blot (WB) of proliferating cell nuclear antigen (PCNA), monoclonal phosphorylate Histone 3 (serine 10) (PH3) and SMP30 marker. Specific dopaminergic differentiation was confirmed by the WB analysis of tyrosine hydroxylase (TH). Cell maturation and neural capability were characterized using specific markers (SYP: synaptophysin and GIRK2: G-protein-regulated ...
Development of novel silk fibroin/polyvinyl alcohol/sol-gel bioactive glass composite matrix by modified layer by layer electrospinning method for bone tissue construct generation.
Biofabrication. 2017 Mar 23;9(1):015028
Authors: Singh BN, Pramanik K
The worldwide occurrence of bone tissue related defects as well as diseases and lack of successful perpetual cure has attracted attention and accelerated exploration of composite scaffolding material with superior bioactivity, osteoinductivity and osteoconductivity properties. Among such biomaterials, silk fibroin and bioglass composite attained special emphasis to develop tissue engineered construct with a hierarchical structure ranging from nano to microscale thereby mimicking bone tissue extracellular matrix. In the present study, a bilayer deposition of natural biopolymer (silk fibroin) and synthetic polymer (polyvinyl alcohol) solution with 58s bioactive glass sol was done by free liquid surface electrospinning and further stabilized through ethanol washing to fabricate novel nanofibrous composite scaffold. The fabricated composite scaffold possesses superior stiffness, hydrophilicity, bioactivity and superior osteogenic potential. The scaffolds were characterized for its fiber diameter distribution, hydrophilicity, tensile strength and apatite forming ability. Field emission scanning electron microscope and transmission electron microscope analysis demonstrate the apatite like particle formation over the scaffold after treatment with simulated body fluid, which improved osteogenicity of the developed scaffold. The biocompatibility, biomineralization and osteogenic potential of composite scaffold were evaluated by cell culture study using cord blood derived mesenchymal stem cells, proving that the developed composite scaffold is biocompatible and possess excellent osteogenic potential for neo bone tissue regeneration. Thus, the ...