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Novel biomaterials and Tissue engineering
  • Supramolecular surface functionalization via catechols for the improvement of cell-material interactions.

    Posted 2017-06-22 10:00:49 dun: Mahammad A. Tafida

    Supramolecular surface functionalization via catechols for the improvement of cell-material interactions. Biomater Sci. 2017 Jun 21;: Authors: Spaans S, Fransen PPKH, Ippel BD, de Bont DFA, Keizer HM, Bax NAM, Bouten CVC, Dankers PYW Abstract Optimization of cell-material interactions is crucial for the success of synthetic biomaterials in guiding tissue regeneration. To do so, catechol chemistry is often used to introduce adhesiveness into biomaterials. Here, a supramolecular approach based on ureido-pyrimidinone (UPy) modified polymers is combined with catechol chemistry in order to achieve improved cellular adhesion onto supramolecular biomaterials. UPy-modified hydrophobic polymers with non-cell adhesive properties are developed that can be bioactivated via a modular approach using UPy-modified catechols. It is shown that successful formulation of the UPy-catechol additive with the UPy-polymer results in surfaces that induce cardiomyocyte progenitor cell adhesion, cell spreading, and preservation of cardiac specific extracellular matrix production. Hence, by functionalizing supramolecular surfaces with catechol functionalities, an adhesive supramolecular biomaterial is developed that allows for the possibility to contribute to biomaterial-based regeneration. PMID: 28636048 [PubMed - as supplied by ...

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  • Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions.

    Posted 2017-06-22 10:00:49 dun: Mahammad A. Tafida

    Related Articles Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions. Methods Mol Biol. 2017;1612:79-94 Authors: Montufar EB, Vojtova L, Celko L, Ginebra MP Abstract The present method describes the procedure to fabricate calcium phosphate foams with suitable open porosity, pore size, and composition to perform three-dimensional (3D) cell cultures with the objective to simulate the bone tissue microenvironment in vitro. Foams with two compositions but equivalent porosity can be fabricated. On the one hand, hydroxyapatite foams obtained by hydrolysis at 37 °C, with microstructure that mimics the small crystal size of the mineral component of bones, and on the other hand, beta tricalcium phosphate foams with polygonal grains obtained by sintering at 1100 °C. In the first part of the chapter the calcium phosphate foams are briefly described. Afterwards, the foaming process is described in detail, including alternatives to overcome processing problems than can arise. Finally, insights are provided on how to perform 3D cell cultures using the calcium phosphate foams as substrates. PMID: 28634936 [PubMed - in ...

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  • Directional Matrix Nanotopography with Varied Sizes for Engineering Wound Healing.

    Posted 2017-06-22 10:00:49 dun: Mahammad A. Tafida

    Directional Matrix Nanotopography with Varied Sizes for Engineering Wound Healing. Adv Healthc Mater. 2017 Jun 21;: Authors: Kim J, Bae WG, Kim YJ, Seonwoo H, Choung HW, Jang KJ, Park S, Kim BH, Kim HN, Choi KS, Kim MS, Choung PH, Choung YH, Chung JH Abstract Topographic features play a crucial role in the regulation of physiologically relevant cell and tissue functions. Here, an analysis of feature-size-dependent cell-nanoarchitecture interactions is reported using an array of scaffolds in the form of uniformly spaced ridge/groove structures for engineering wound healing. The ridge and groove widths of nanopatterns are varied from 300 to 800 nm and the nanotopography features are classified into three size ranges: dense (300-400 nm), intermediate (500-600 nm), and sparse (700-800 nm). On these matrices, fibroblasts demonstrate a biphasic trend of cell body and nucleus elongation showing the maximum at intermediate feature density, whereas maximum migration speed is observed at the dense case with monotonic decrease upon increasing feature size. The directional organization of cell-synthesized fibronectin fibers can be regulated differently via the nanotopographical features. In an in vitro wound healing model, the covering rate of cell-free regions is maximized on the dense nanotopography and decreased with increasing feature size, showing direct correlation with the trend of migration speed. It is demonstrated that the properties of repaired tissue matrices in the process of wound healing may be controlled via the feature-size-dependent cell-nanoarchitecture interactions, which can be an important consideration for designing tissue engineering scaffolds. PMID: 28636203 [PubMed - as supplied by ...

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  • Engineering Human Bone Grafts with New Macroporous Calcium Phosphate Cement Scaffolds.

    Posted 2017-06-22 10:00:49 dun: Mahammad A. Tafida

    Related Articles Engineering Human Bone Grafts with New Macroporous Calcium Phosphate Cement Scaffolds. J Tissue Eng Regen Med. 2017 Jun 21;: Authors: Sladkova M, Palmer M, Öhman C, Cheng J, Al-Ansari S, Saad M, Engqvist H, de Peppo GM Abstract Bone engineering opens the possibility to grow large amounts of tissue products by combining patient-specific cells with compliant biomaterials. Decellularized tissue matrices represent suitable biomaterials but availability, long processing time, excessive cost, and concerns on pathogen transmission have led to the development of biomimetic synthetic alternatives. We recently fabricated calcium phosphate cement (CPC) scaffolds with variable macroporosity using a facile synthesis method with minimal manufacturing steps, and demonstrated long-term biocompatibility in vitro. However, there is no knowledge on the potential use of these scaffolds for bone engineering, and whether the porosity of the scaffolds affects osteogenic differentiation and tissue formation in vitro. In this study we explored the bone engineering potential of CPC scaffolds with two different macroporosities using human mesenchymal progenitors derived from induced pluripotent stem cells (iPSC-MP) or isolated from bone marrow (BMSC). Biomimetic decellularized bone scaffolds were used as reference material in all experiments. The results demonstrate that, irrespective of their macroporosity, the CPC scaffolds tested in this study support attachment, viability and growth of iPSC-MP and BMSC cells similarly to decellularized bone. Importantly, the tested materials sustained differentiation of the cells as evidenced by increased expression of osteogenic markers and formation of a mineralized tissue. In conclusion, the results of this study suggest that the CPC scaffolds fabricated using our method are suitable to engineer bone grafts from different cell sources, and could lead to development of safe and ...

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  • The fabrication of iron oxide nanoparticle-nanofiber composites by electrospinning and their applications in tissue engineering.

    Posted 2017-06-22 10:00:49 dun: Mahammad A. Tafida

    Related Articles The fabrication of iron oxide nanoparticle-nanofiber composites by electrospinning and their applications in tissue engineering. Biotechnol J. 2017 Jun 21;: Authors: Mortimer CJ, Wright CJ Abstract This paper reviews the use of iron oxide nanoparticle-nanofiber composites in tissue engineering with a focus on the electrospinning technique. Electrospinning is an established method of scaffold fabrication offering a number of key advantages which include its facile nature, with electrospun materials offering a high surface area to volume ratio, potential for the release of drugs and antimicrobials, controllable fiber diameters and high porosity and permeability. A number of different techniques for the preparation of iron oxide nanoparticles including their functionalization are discussed along with their applications in the biomedical field. The review then focusses on the fabrication of nanoparticle-nanofiber composite scaffolds formed using electrospinning. The advantages and disadvantages of current fabrication techniques are discussed including the fabrication of nanofibers using pre-synthesized nanoparticles and post-treatment synthesized nanoparticles. We demonstrate that emerging in-situ synthesis techniques show promise by offering a reduced number of steps and simpler procedures for the production of magnetic scaffolds. These scaffolds have a number of applications in tissue engineering, allowing for improved bone and tissue repair. PMID: 28635132 [PubMed - as supplied by ...

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